PE Anti-Mouse CD40 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using PE-98122

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3), and 0.25 ug PE Anti-Mouse CD40 Rabbit RecAb (PE-98122, Clone: 240874G8) or 0.25 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-98122

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse CD40 Rabbit RecAb (PE-98122, Clone: 240874G8) or 0.25 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were not fixed.

CD40 Uni-rAb^TM Recombinant Antibody for FC

Cat No. PE-98122

Clone No.240874G8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

240874G8, Bp50, CD40 antigen, Tnfrsf5

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-98122 targets CD40 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD40 fusion protein Eg1118
• Full Name: CD40 antigen
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_011611.2
• Gene Symbol: Cd40
• Gene ID (NCBI): 21939
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27512-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).