Biotin-conjugated CD40L/CD154 Monoclonal antibody

CD40L/CD154 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

1E6D10

Cat no : Biotin-66502

Synonyms

CD154, CD40L; CD154, CD40LG, CD40 ligand, CD40 L

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Validation Data Gallery

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  IHC staining of human tonsillitis using Biotin-66502

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66502 (CD40L/CD154 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-66502

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66502 (CD40L/CD154 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66502 targets CD40L/CD154 in IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD40L/CD154 fusion protein Ag24941
• Full Name: CD40 ligand
• Calculated Molecular Weight: 261 aa, 29 kDa
• GenBank Accession Number: BC071754
• Gene Symbol: CD40 Ligand
• Gene ID (NCBI): 959
• RRID: AB_2883075
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kDa transmembrane homologue or 18-kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.