Anti-Human CD40L/CD154 Rabbit scFv Antibody

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• Anti-Human CD40L/CD154 Rabbit scFv Antibody (SF98355-2-RR)

CD40L/CD154 Recombinant Antibody for FC

Cat No. SF98355-2-RR

Clone No.242847F7

Host / Type

Rabbit / scFv

Reactivity

human

Applications

FC

Synonyms

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

• 

  FC experiment of human PBMCs using SF98355-2-RR

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit scFv Antibody (SF98355-2-RR, Clone: 242847F7) and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were then stained with APC Anti-Human CD4. Cells were incubated with FC Receptor Block before staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using SF98355-2-RR

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit scFv Antibody (SF98355-2-RR, Clone: 242847F7)(red), or 0.25 ug Rabbit scFv Isotype Control Antibody (SF98136-1-RR, Clone: 240953C9)(blue) and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit scFv Antibody (SF98355-2-RR, Clone: 242847F7)(black) and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1)(black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: PMA and ionomycin treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF98355-2-RR targets CD40L/CD154 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg3501

  Product name: Recombinant Human CD40L/CD154 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 113-261 aa of BC071754

  Sequence: MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

• Full Name: CD40 ligand
• Calculated Molecular Weight: 261 aa, 29 kDa
• GenBank Accession Number: BC071754
• Gene Symbol: CD40 Ligand
• Gene ID (NCBI): 959
• RRID: AB_3750160
• Conjugate: Unconjugated
• Source: Anti-Human CD40L/CD154 scFv from clone 242847F7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P29965
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.