CD43 Monoclonal antibody
CD43 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2A11D6
Cat no : 66224-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells |
Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Jurkat cells, human tonsillitis tissue |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66224-1-Ig targets CD43 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD43 fusion protein Ag23595 |
Full Name | sialophorin |
Calculated molecular weight | 400 aa, 43 kDa |
Observed molecular weight | 115 kDa |
GenBank accession number | BC012350 |
Gene symbol | SPN |
Gene ID (NCBI) | 6693 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.
Protocols
Product Specific Protocols | |
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WB protocol for CD43 antibody 66224-1-Ig | Download protocol |
IHC protocol for CD43 antibody 66224-1-Ig | Download protocol |
IF protocol for CD43 antibody 66224-1-Ig | Download protocol |
FC protocol for CD43 antibody 66224-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |