CD44 Recombinant antibody

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  WB analysis using 85163-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85163-5-RR (Cd44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85163-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85163-5-RR (Cd44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 85163-5-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Cd44 antibody (85163-5-RR, Clone: 242666C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD44 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85163-5-RR

Clone No.242666C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

ECMR-III, ECMR III, CSPG8, CDw44, CD44 antigen

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Tested Applications

• Positive WB detected in: C2C12 cells, HeLa cells, RAW 264.7 cells, A549 cells
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85163-5-RR targets CD44 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD44 antigen
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: NM_009851.2
• Gene Symbol: Cd44
• Gene ID (NCBI): 12505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: NP_033981
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.