CoraLite® Plus 488-conjugated CD44 Recombinant monoclonal antibody

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  IF Staining of rat spleen using CL488-84369-5

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CoraLite® Plus 488 CD44 antibody (CL488-84369-5, Clone: 241648D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat spleen using CL488-84369-5

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CoraLite® Plus 488 CD44 antibody (CL488-84369-5, Clone: 241648D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD44 Uni-rAb^® Recombinant Antibody for IF-P

Cat No. CL488-84369-5

Clone No.241648D1

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

IF-P

Synonyms

241648D1, CD44 antigen, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor

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Tested Applications

• Positive IF-P detected in: rat spleen tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84369-5 targets CD44 in IF-P applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1458

  Product name: Recombinant Rat CD44 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-271 aa of XM_006234631.3

  Sequence: QQQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCEAFNTTLPTMAQMELALSKGFETCRYGFIEGHVVIPRIHPNAICAANNTGVYILLASNTSHYDTYCFNASAPLEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDEDVSSGSTIEKSTPEGYILHTDLPTSQPTGDRDDAFFIGSTLATSDGDSSMDPRGGFDTVTHGSELAGHSSGNQDSGVTTTSGPARRPQIPE

• Full Name: Cd44 molecule
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: XM_006234631.3
• Gene Symbol: Cd44
• Gene ID (NCBI): 25406
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O70509
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.