CoraLite® Plus 488-conjugated CD44 Recombinant antibody

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  IF Staining of HepG2 using CL488-84854-5

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CD44 antibody (CL488-84854-5, Clone: 242345G11 ) at dilution of 1:200.

CD44 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84854-5

Clone No.242345G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242345G11, CD44 antigen, CDW44, CSPG8, ECMR III

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84854-5 targets CD44 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD44 fusion protein Eg1057
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: NM_000610.3
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• ENSEMBL Gene ID: ENSG00000026508
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16070-1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.