FcZero-rAb® CoraLite® Plus 647 Anti-Rat CD44 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 647 Anti-Rat CD44 Rabbit Recombinant Antibody (CL647-FcA98208)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD44 FcZero-rAb^® Recombinant Antibody for FC

Cat No. CL647-FcA98208 Copied!

Clone No. 241648F8

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Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

CD44 antigen, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen

• 

  FC experiment of rat splenocytes using CL647-FcA98208

  1x10^6 rat splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Rat CD44 Rabbit RecAb (CL647-FcA98208, Clone: 241648F8) (red) or 0.25 ug FcZero-rAb® CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-FcA98208 targets CD44 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1458

  Product name: Recombinant Rat CD44 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-271 aa of XM_006234631.3

  Sequence: QQQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCEAFNTTLPTMAQMELALSKGFETCRYGFIEGHVVIPRIHPNAICAANNTGVYILLASNTSHYDTYCFNASAPLEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDEDVSSGSTIEKSTPEGYILHTDLPTSQPTGDRDDAFFIGSTLATSDGDSSMDPRGGFDTVTHGSELAGHSSGNQDSGVTTTSGPARRPQIPE

• Full Name: Cd44 molecule
• Calculated Molecular Weight: 40 kDa
• GenBank Accession Number: XM_006234631.3
• Gene Symbol: Cd44
• Gene ID (NCBI): 25406
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O70509
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.