CD45 Monoclonal antibody

CD45 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4E9B2

Cat no : 60287-1-Ig

Synonyms

B220, CD45, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive WB detected inpig spleen tissue, Raji cells
Positive IHC detected inhuman appendicitis tissue, human liver cancer tissue, human tonsillitis tissue, human colon tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, mouse spleen tissue
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60287-1-Ig targets CD45 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Cited Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD45 fusion protein Ag13712
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596). 

What is the molecular weight of CD45?

The molecular weight of CD45 is a 180-240 kDa. 

What are the isoforms of CD45?

Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). 

What is the tissue specificity of CD45?

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). 

What are the post-translational modifications of CD45?

CD45 can be heavily N- and O-glycosylated (PMID: 1702721). 

What is CD45's involvement in disease?

Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).


Protocols

Product Specific Protocols
WB protocol for CD45 antibody 60287-1-IgDownload protocol
IHC protocol for CD45 antibody 60287-1-IgDownload protocol
IF protocol for CD45 antibody 60287-1-IgDownload protocol
FC protocol for CD45 antibody 60287-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC

Hepatobiliary Pancreat Dis Int

Hydrogen-rich saline protects against hepatic injury induced by ischemia-reperfusion and laparoscopic hepatectomy in swine.

Authors - Yan-Song Ge

Cell Reprogram

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Authors - Qi Li
humanFC

Exp Cell Res

Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.

Authors - Xun Xi
mouseWB

Ecotoxicol Environ Saf

PM2.5-induced lung injury is attenuated in macrophage-specific NLRP3 deficient mice.

Authors - Rui Xiong
mouseIHC

Stem Cell Reports

Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation.

Authors - Hitomi Matsunari
mouseIHC

Cell Biol Toxicol

Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.

Authors - Yiming Jiang