CD45 Monoclonal antibody

CD45 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4E9B2

Cat no : 60287-1-Ig

Synonyms

B220, CD45, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive WB detected inpig spleen tissue, Raji cells
Positive IHC detected inhuman appendicitis tissue, human colon tissue, human liver cancer tissue, human tonsillitis tissue, mouse spleen tissue, rat colon tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, mouse spleen tissue
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60287-1-Ig targets CD45 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD45 fusion protein Ag13712
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596). 

What is the molecular weight of CD45?

The molecular weight of CD45 is a 180-240 kDa. 

What are the isoforms of CD45?

Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). 

What is the tissue specificity of CD45?

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). 

What are the post-translational modifications of CD45?

CD45 can be heavily N- and O-glycosylated (PMID: 1702721). 

What is CD45's involvement in disease?

Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).


Protocols

Product Specific Protocols
WB protocol for CD45 antibody 60287-1-IgDownload protocol
IHC protocol for CD45 antibody 60287-1-IgDownload protocol
IF protocol for CD45 antibody 60287-1-IgDownload protocol
FC protocol for CD45 antibody 60287-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC

Hepatobiliary Pancreat Dis Int

Hydrogen-rich saline protects against hepatic injury induced by ischemia-reperfusion and laparoscopic hepatectomy in swine.

Authors - Yan-Song Ge
humanIHC

J Oncol

Analysis of Clinical Characteristics, Radiological Predictors, Pathological Features, and Perioperative Outcomes Associated with Perinephric Fat Adhesion Degree

Authors - Junqiang Liu
mouseFC

Adv Sci (Weinh)

A Long-Term Clearing Cranial Window for Longitudinal Imaging of Cortical and Calvarial Ischemic Injury through the Intact Skull.

Authors - Chao Zhang

Cell Reprogram

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Authors - Qi Li
humanFC

Exp Ther Med

Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.

Authors - Junjie Liu
humanIF

J Dermatol

Reduced expression of programmed cell death 1 and programmed cell death ligand 1 in infiltrating inflammatory cells of lichen planus without administration of immune checkpoint inhibitors.

Authors - Kazufumi Shirouchi