FcZero-rAb™ Biotin Anti-Human CD45 Rabbit Recombinant Antibody

• 

  FC experiment of human peripheral blood leukocyte using Biotin-FcA98117

  1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Biotin Anti-Human CD45 Rabbit RecAb (Biotin-FcA98117, Clone: 241670E10) or 0.25 ug FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and PE Streptavidin (Cat.No. PF00021). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using Biotin-FcA98117

  1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Biotin Anti-Human CD45 Rabbit RecAb (Biotin-FcA98117, Clone: 241670E10) (red) or 0.25 ug FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (bue), and PE Streptavidin (Cat.No. PF00021). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD45 FcZero-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. Biotin-FcA98117

Clone No.241670E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

241670E10, EC:3.1.3.48, GP180, L CA, LCA

---

Tested Applications

• Positive FC (Intra) detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98117 targets CD45 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2486

  Product name: recombinant human CD45(220-577) protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007019 of

  Sequence:

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 147 kDa
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08575
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).