CoraLite®488 Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for IF,FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

IF,FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2D1

Cat no : CL488-65082

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive IF detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65082-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65082 targets CD45 in IF,FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human PBMC
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
IF protocol for CL488 CD45 antibody CL488-65082Download protocol
FC protocol for CL488 CD45 antibody CL488-65082Download protocol
Standard Protocols
Click here to view our Standard Protocols