PE Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF, FC

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

2D1

Cat no : PE-65082

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using PE-65082

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug PE-Anti-Human CD45 (PE-65082, clone 2D1) (red) or 0.5 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.

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  FC experiment of Raji using PE-65082

  1X10^6 Raji cells were surface stained with 5 ul PE Anti-Human CD45 (PE-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

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Tested Applications

• Positive FC detected in: Human peripheral blood lymphocytes, Raji cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

PE-65082 targets CD45 in IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human PBMC
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• RRID: AB_2883877
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
human	IF	Exp Ther Med; Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer; Authors - Jianping Jiang