Home
Products
Primary Antibodies
Recombinant Antibodies
Mouse CD45 Recombinant monoclonal antibody, PBS Only (Capture) (83396-14-PBS)

Mouse CD45 Recombinant monoclonal antibody, PBS Only (Capture)

Name: Mouse CD45 Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 83396-14-PBS-100UG

CD45 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 83396-14-PBS

Clone No. 240920B4

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

Ptprc, EC:3.1.3.48, LCA, L-CA, Leukocyte common antigen

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllSandwich ELISA sample testSandwich ELISAAffinity

Sandwich ELISA sample test of MP00412-4
The mean mouse CD45 concentration was determined to be 39.3 ng/mL in RAW 264.7 cell extract based on a 4.8 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP00412-4
RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD45, and measured 154.9 pg/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA standard curve of MP00412-4
Sandwich ELISA standard curve of MP00412-4, Mouse CD45 Recombinant Matched Antibody Pair - PBS only. 83396-14-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0845. 83396-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 83396-14-PBS
Biolayer interferometry (BLl) kinetic assays of 83396-14-PBS against Mouse CD45 were performed. The affinity constant is 1.89 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CD45 in ?

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

83396-14-PBS targets CD45 as part of a matched antibody pair:

MP00412-4: 83396-14-PBS capture and 83396-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0845 Product name: Recombinant mouse CD45/PTPRC protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 26-566 aa of NM_001111316.2 Sequence: QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK Predict reactive species
Full Name	protein tyrosine phosphatase, receptor type, C
Calculated Molecular Weight	145 kDa
GenBank Accession Number	NM_001111316.2
Gene Symbol	CD45
Gene ID (NCBI)	19264
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P06800-4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.