PE Anti-Rat CD45RA (OX-33)

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  FC experiment of rat splenocytes using PE-65234

  1x10^6 rat splenocytes were surface stained with 0.1 ug PE Anti-Rat CD45RA (OX-33) (PE-65234, Clone: OX-33) or 0.1 ug PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21), and APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were not fixed.

CD45RA Monoclonal Antibody for FC

Cat No. PE-65234

Clone No.OX-33

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

FC

Synonyms

CD45, Lca, Ptprc, L CA, OX-33

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65234 targets CD45RA in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Leukocyte common antigen purified from rat splenocytes

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: CD45
• Gene ID (NCBI): 24699
• RRID: AB_3673995
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P04157
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).