Anti-Human CD45RO (UCHL1), PBS Only

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  IHC staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CD45RO antibody (65150-1-Ig, Clone: UCHL1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

  1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD45RA and 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

CD45RO Monoclonal Antibody for IHC, IF-P, FC

Cat No. 65150-1-PBS

Clone No.UCHL1

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IHC, IF-P, FC

Synonyms

CD45, EC:3.1.3.48, GP180, L CA, LCA

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Product Information

65150-1-PBS targets CD45RO in IHC, IF-P, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  IL-2 dependent T cell line, CA1

• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_2881476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08575
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).