FcZero-rAb® Biotin Anti-Human CD46 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human CD46 Rabbit Recombinant Antibody (Biotin-FcA98240)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD46 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98240

Clone No.241764C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

MCP, Membrane cofactor protein, MIC10, TLX, TRA2.10

• 

  FC experiment of human PBMCs using Biotin-FcA98240

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD46 Rabbit RecAb (Biotin-FcA98240, Clone:241764C9), or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) and Streptavidin-PE Conjugate (PE-PF00030). Cells were then stained with FcZero-rAb® APC Anti-Human CD19 (4G7) Rabbit Recombinant Antibody (APC-FcA65562, Clone: 4G7). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using Biotin-FcA98240

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD46 Rabbit RecAb (Biotin-FcA98240, Clone:241764C9) (red), or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue) and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98240 targets CD46 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1770

  Product name: Recombinant Human CD46 protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 35-328 aa of NM_172351.3

  Sequence: CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLD

• Full Name: CD46 molecule, complement regulatory protein
• Calculated Molecular Weight: 42kDa
• GenBank Accession Number: NM_172351.3
• Gene Symbol: CD46
• Gene ID (NCBI): 4179
• RRID: AB_3746992
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15529-11
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).