CoraLite® Plus 488-conjugated CD46 Recombinant antibody

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  IF Staining of HeLa using CL488-84599-5

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CD46 antibody (CL488-84599-5, Clone: 241764F8 ) at dilution of 1:200.

CD46 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84599-5

Clone No.241764F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241764F8, MCP, Membrane cofactor protein, MIC10, TLX

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84599-5 targets CD46 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD46 fusion protein Eg1770
• Full Name: CD46 molecule, complement regulatory protein
• Calculated Molecular Weight: 42kDa
• Observed Molecular Weight: 50-65 kDa
• GenBank Accession Number: NM_172351.3
• Gene Symbol: CD46
• Gene ID (NCBI): 4179
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15529-11
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).