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Mouse CD49b Recombinant monoclonal antibody, PBS Only (87503-1-PBS)

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WB analysis using 87503-1-RR (same clone as 87503-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87503-1-RR (ITA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87503-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 87503-1-RR (same clone as 87503-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87503-1-RR (CD49b antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87503-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 87503-1-RR (same clone as 87503-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87503-1-RR (CD49b antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87503-1-PBS in a different storage buffer formulation.
Affinity of 87503-1-RR
Biolayer interferometry (BLl) kinetic assays of 87503-1-RR against Mouse CD49b were performed. The affinity constant is 17.7 nM.

Mouse CD49b Recombinant monoclonal antibody, PBS Only

Name: Mouse CD49b Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 87503-1-PBS

CD49b Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 87503-1-PBS

Clone No.252736G5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Itga2, DX5, integrin alpha 2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87503-1-PBS targets CD49b in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg10475 Product name: Recombinant mouse ITA2 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Domain: 10011440 of Sequence: \ Predict reactive species
Full Name	integrin alpha 2
Observed Molecular Weight	150-160 kDa
Gene Symbol	CD49b
Gene ID (NCBI)	16398
RRID	AB_3745585
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q62469
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.