CD49b Recombinant monoclonal antibody

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• CD49b Recombinant monoclonal antibody (87503-1-RR)

• 

  WB analysis using 87503-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87503-1-RR (ITA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 87503-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87503-1-RR (CD49b antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 87503-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87503-1-RR (CD49b antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity of 87503-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87503-1-RR against Mouse CD49b were performed. The affinity constant is 17.7 nM.

CD49b Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87503-1-RR

Clone No.252736G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Itga2, DX5, integrin alpha 2

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Tested Applications

• Positive WB detected in: A549 cells, mouse spleen tissue, rat spleen tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87503-1-RR targets CD49b in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10475

  Product name: Recombinant mouse ITA2 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011440 of

  Sequence: \

• Full Name: integrin alpha 2
• Observed Molecular Weight: 150-160 kDa
• Gene Symbol: CD49b
• Gene ID (NCBI): 16398
• RRID: AB_3745585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62469
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.