CoraLite®700 Anti-Human CD49b (AK7) Mouse IgG2a Recombinant Antibody

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  FC experiment of human peripheral blood platelets using CL700-65583

  1x10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite®700 Anti-Human CD49b (AK7) Mouse IgG2a RecAb (CL700-65583, Clone: AK7) (red) or unstained (blue). Cells were not fixed.

CD49b Recombinant Antibody for FC

Cat No. CL700-65583

Clone No.AK7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

ITGA2, AK7, BR, Collagen receptor, GPIa

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL700-65583 targets CD49b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human platelets
• Full Name: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Calculated Molecular Weight: 126 kDa
• GenBank Accession Number: NM_002203
• Gene Symbol: Integrin alpha 2
• Gene ID (NCBI): 3673
• Conjugate: CoraLite®700 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 702 nm / 723 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17301
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.