Anti-Human Integrin alpha 6 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood lymphocytes using 98257-1-RR

  1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD49f/Integrin alpha 6 Rabbit Recombinant Antibody (98257-1-RR, Clone: 241703B5) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD61. Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using 98257-1-RR

  1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD49f/Integrin alpha 6 Rabbit Recombinant Antibody (98257-1-RR, Clone: 241703B5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98257-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98257-1-RR against Human CD49f/Integrin alpha 6 were performed. The affinity constant is 0.400 nM.

Integrin alpha 6 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98257-1-RR

Clone No.241703B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ITGA6, 241703B5, CD49 antigen-like family member F, CD49f, Integrin alpha-6

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98257-1-RR targets Integrin alpha 6 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: integrin, alpha 6
• Calculated Molecular Weight: 1073 aa, 119 kDa
• GenBank Accession Number: BC136456
• Gene Symbol: Integrin alpha 6
• Gene ID (NCBI): 3655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P23229
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).