CoraLite® Plus 647 Anti-Human Integrin alpha 6 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood lymphocytes using CL647-98257

  1x10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD61, and 5 ul CoraLite® Plus 647 Anti-Human Integrin alpha 6 Rabbit RecAb (CL647-98257, Clone: 241703B5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL647-98257

  1x10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human Integrin alpha 6 Rabbit RecAb (CL647-98257, Clone: 241703B5) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

Integrin alpha 6 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98257

Clone No.241703B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ITGA6, 241703B5, CD49 antigen-like family member F, CD49f, Integrin alpha-6

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98257 targets Integrin alpha 6 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: integrin, alpha 6
• Calculated Molecular Weight: 1073 aa, 119 kDa
• GenBank Accession Number: BC136456
• Gene Symbol: Integrin alpha 6
• Gene ID (NCBI): 3655
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23229
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).