CD5 Recombinant antibody

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  WB analysis of EL-4 using 83451-4-RR

  EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 83451-4-RR

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 83451-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83451-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 83451-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD5 antibody (83451-4-RR, Clone: 240244C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83451-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83451-4-RR against Mouse CD5 were performed. The affinity constant is 89.9 pM.

CD5 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83451-4-RR

Clone No.240244C2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

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Tested Applications

• Positive WB detected in: EL-4 cells, mouse thymus tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83451-4-RR targets CD5 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD5 antigen
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55 kDa, 67 kDa
• GenBank Accession Number: NM_007650.3
• Gene Symbol: Cd5
• Gene ID (NCBI): 12507
• RRID: AB_3671088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13379
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).