FC experiment of mouse splenocytes using 98038-2-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed.
FC experiment of mouse splenocytes using 98038-2-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Affinity and Kinetic Characterization of 98038-2-RR
Biolayer interferometry (BLl) kinetic assays of 98038-2-RR against Mouse CD5 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98038-2-RR targets CD5 in FC applications and shows reactivity with Mouse samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).
FC experiment of mouse splenocytes using 98038-2-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed.
FC experiment of mouse splenocytes using 98038-2-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98038-2-RR
Biolayer interferometry (BLl) kinetic assays of 98038-2-RR against Mouse CD5 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CD5 Recombinant antibody
Catalog Number
98038-2-RR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
