CoraLite®594 Anti-Mouse CD5 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL594-98038-2

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 and 0.25 ug CoraLite®594 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL594-98038-2, Clone: 240244A7), or Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of mouse splenocytes using CL594-98038-2

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL594-98038-2, Clone: 240244A7) (red), or Isotype Control (blue). Cells were not fixed.

CD5 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL594-98038-2

Clone No.240244A7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98038-2 targets CD5 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 antigen
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: NM_007650.3
• Gene Symbol: Cd5
• Gene ID (NCBI): 12507
• RRID: AB_3673556
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13379
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).