CoraLite®488 Anti-Human CD5 (L17F12)

CD5 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

L17F12

Cat no : CL488-65200

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65200-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65200 targets CD5 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Human T-cell acute lymphoblastic leukemia (T-ALL) cells
Full Name CD5 molecule
Calculated molecular weight 495 aa, 55 kDa
GenBank accession numberBC027901
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
FC protocol for CL488 CD5 antibody CL488-65200Download protocol
Standard Protocols
Click here to view our Standard Protocols