PE Anti-Human CD5 (UCHT2)

• 

  FC experiment of human PBMCs using PE-65152

  1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD19 and 5 ul PE Anti-Human CD5 (PE-65152, Clone:UCHT2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65152

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD5 (PE-65152, Clone:UCHT2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD5 Monoclonal Antibody for FC

Cat No. PE-65152

Clone No.UCHT2

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65152 targets CD5 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: CD5 molecule
• Calculated Molecular Weight: 495 aa, 55 kDa
• GenBank Accession Number: BC027901
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• ENSEMBL Gene ID: ENSG00000110448
• RRID: AB_2883931
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P06127
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).