CoraLite® Plus 647-conjugated CD53 Recombinant antibody

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  IF Staining of Jurkat using CL647-84837

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 647 CD53 antibody (CL647-84837, Clone: 242038C5 ) at dilution of 1:200.

CD53 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL647-84837

Clone No.242038C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-84837 targets CD53 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD53 fusion protein Eg2182
• Full Name: CD53 molecule
• Calculated Molecular Weight: 24kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: NM_000560.4
• Gene Symbol: CD53
• Gene ID (NCBI): 963
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19397
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).