Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody

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• Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR)

ICAM-1/CD54 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98049-1-RR Copied!

Clone No. 240249D10

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD54, Icam-1, MALA-2, Icam1, ICAM 1

• 

  FC experiment of mouse splenocytes using 98049-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR, Clone:240249D10) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of mouse splenocytes using 98049-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR, Clone:240249D10)(red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98049-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98049-1-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.49 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98049-1-RR targets ICAM-1/CD54 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• RRID: AB_3672196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13597
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Publications

What published studies show

The Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (Catalog No. 98049-1-RR) has a total of 2 citations. These appear in Advanced Healthcare Materials and Frontiers in Cellular and Infection Microbiology. The associated research fields include development of bioresponsive nanoparticles with antimicrobial activity for treating bacterial infections, and investigation of how Veillonella parvula outer membrane vesicles increase ICAM-1⁺ neutrophils with elevated NET formation via ROS-PAD4 signaling.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	FC	Adv Healthc Mater; De Novo Development of Bioresponsive Nanoparticles With Antimicrobial Activity to Treat Bacterial Infections.; Authors - Liang Zhao
mouse		Front Cell Infect Microbiol; Veillonella parvula outer membrane vesicles increase ICAM-1+ neutrophils exhibiting elevated NET formation via ROS-PAD4 signaling; Authors - Lina Xu