Anti-Mouse ICAM-1/CD54 (YN1/1.7.4)

ICAM-1/CD54 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

YN1/1.7.4

Cat no : 65066-1-Ig

Synonyms

CD54, Icam-1, YN1/1.7.4, MALA-2, Icam1

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65066-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD54 (65066-1-Ig, Clone: YN1/1.7.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65066-1-Ig targets ICAM-1/CD54 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse NS-1 cells
• Full Name: intercellular adhesion molecule 1
• GenBank Accession Number: BC008626
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• RRID: AB_2918373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).