CoraLite® Plus 488 Anti-Mouse CD54 (YN1/1.7.4)

CD54 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

YN1/1.7.4

Cat no : CL488-65066

Synonyms

CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65066 targets CD54 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen Mouse NS-1 cells
Full Name intercellular adhesion molecule 1
GenBank Accession NumberBC008626
Gene Symbol Icam1
Gene ID (NCBI) 15894
RRIDAB_2883159
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD54 antibody CL488-65066Download protocol
Standard Protocols
Click here to view our Standard Protocols