CoraLite® Plus 488 Anti-Human NCAM1/CD56 (B-A19)

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  FC experiment of human PBMCs using CL488-65264

  1X10^6 human PBMCs were surface co-stained with CL750 Anti-human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD56 (CL488-65264, Clone:B-A19), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65264

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD56 (CL488-65264, Clone:B-A19), or Mouse IgG1 Isotype Control. Cells were not fixed. CD3-negative lymphocytes were gated.

NCAM1/CD56 Monoclonal Antibody for FC

Cat No. CL488-65264

Clone No.B-A19

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Synonyms

CD56, NCAM1, NCAM 1, NCAM, N CAM 1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65264 targets NCAM1/CD56 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: neural cell adhesion molecule 1
• GenBank Accession Number: BC014205
• Gene Symbol: NCAM1
• Gene ID (NCBI): 4684
• ENSEMBL Gene ID: ENSG00000149294
• RRID: AB_3084187
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P13591
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.