Anti-Human CD58 (TS2/9)

CD58 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/9

Cat no : 65193-1-Ig

Synonyms

Ag3, CD58, CD58 molecule, FLJ23181, FLJ43722, LFA 3, LFA3, Surface glycoprotein LFA 3

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65193-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65193-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65193-1-Ig targets CD58 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human cytolytic T cells
• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• RRID: AB_2918463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.