Filter:
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL405-65193 targets CD58 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human cytolytic T cells Predict reactive species |
| Full Name | CD58 molecule |
| Calculated Molecular Weight | 28 kDa |
| GenBank Accession Number | BC005930 |
| Gene Symbol | CD58 |
| Gene ID (NCBI) | 965 |
| RRID | AB_3083840 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19256 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD58 antibody CL405-65193 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
