FcZero-rAb® Biotin Anti-Human CD58 (TS2/9) Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human CD58 (TS2/9) Rabbit Recombinant Antibody (Biotin-FcA65597)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD58 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA65597 Copied!

Clone No. TS2/9

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3

• 

  FC experiment of human peripheral blood leukocytes using Biotin-FcA65597

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD58 (TS2/9) Rabbit RecAb (Biotin-FcA65597, Clone: TS2/9) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human peripheral blood leukocytes using Biotin-FcA65597

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD58 (TS2/9) Rabbit RecAb (Biotin-FcA65597, Clone: TS2/9) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65597 targets CD58 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human cytolytic T cells

• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• RRID: AB_3746826
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19256
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.