FITC Plus Anti-Human CD59 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using FITC-98099

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 (OKT3), and 5 ul FITC Plus Anti-Human CD59 Rabbit RecAb (FITC-98099, Clone: 241311A4) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98099

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD59 Rabbit RecAb (FITC-98099, Clone: 241311A4) (red) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD59 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98099

Clone No.241311A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

20 kDa homologous restriction factor, 241311A4, CD 59, CD59 antigen, CD59 glycoprotein

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98099 targets CD59 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13987
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.