CD5L Recombinant monoclonal antibody

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• CD5L Recombinant monoclonal antibody (86638-1-RR)

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  WB analysis of human plasma using 86638-1-RR

  human plasma were subjected to SDS PAGE followed by western blot with 86638-1-RR (CD5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected CHO-K1 using 86638-1-RR

  Immunofluorescent analysis of (4% PFA) fixed transfected CHO-K1 using CD5L antibody (86638-1-RR, Clone: 250651G1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  Affinity and Kinetic Characterization of 86638-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86638-1-RR against Human CD5L were performed. The affinity constant is 0.487 nM.

CD5L Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86638-1-RR

Clone No.250651G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

AIM, API6, Apoptosis inhibitor expressed by macrophages, CD5 antigen-like, CT-2

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Tested Applications

• Positive WB detected in: human plasma
• Positive IF/ICC detected in: Transfected CHO-K1

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86638-1-RR targets CD5L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3129

  Product name: Recombinant Human CD5L protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 20-347 aa of NM_005894.2

  Sequence: SPSGVRLVGGLHRCEGRVEVEQKGQWGTVCDDGWDIKDVAVLCRELGCGAASGTPSGILYEPPAEKEQKVLIQSVSCTGTEDTLAQCEQEEVYDCSHDEDAGASCENPESSFSPVPEGVRLADGPGHCKGRVEVKHQNQWYTVCQTGWSLRAAKVVCRQLGCGRAVLTQKRCNKHAYGRKPIWLSQMSCSGREATLQDCPSGPWGKNTCNHDEDTWVECEDPFDLRLVGGDNLCSGRLEVLHKGVWGSVCDDNWGEKEDQVVCKQLGCGKSLSPSFRDRKCYGPGVGRIWLDNVRCSGEEQSLEQCQHRFWGFHDCTHQEDVAVICSG

• Full Name: CD5 molecule-like
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_005894.2
• Gene Symbol: CD5L
• Gene ID (NCBI): 922
• RRID: AB_3745022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43866
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).