CoraLite® Plus 647 Anti-Mouse CD6 Rabbit Recombinant Antibody

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• CoraLite® Plus 647 Anti-Mouse CD6 Rabbit Recombinant Antibody (CL647-98161)

CD6 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL647-98161 Copied!

Clone No. 241471F3

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241471F3, T-cell differentiation antigen CD6

• 

  FC experiment of BALB/c mouse splenocytes using CL647-98161

  1x10^6 BALB/c mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3 (17A2) (CL488-65077, Clone:17A2), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD6 Rabbit RecAb (CL647-98161, Clone: 241471F3) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of BALB/c mouse splenocytes using CL647-98161

  1x10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD6 Rabbit RecAb (CL647-98161, Clone: 241471F3) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: BALB/c mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98161 targets CD6 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1401

  Product name: Recombinant Mouse CD6 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-396 aa of NM_009852.3

  Sequence: GLPSPAPSGQHKNGTIPNMTLDLEERLGIRLVNGSSRCSGSVKVLLESWEPVCAAHWNRAATEAVCKALNCGDSGKVTYLMPPTSELPPGATSGNTSSAGNTTWARAPTERCRGANWQFCKVQDQECSSDRRLVWVTCAENQAVRLVDGSSRCAGRVEMLEHGEWGTVCDDTWDLQDAHVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGIDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCRSLGCGSAVARPRGLPHSLDGRMYYSCKGQEPALSTCSWRFNNSNLCSQSRAARVVCSGSQRHLNLSTSEVPSRVPVTIESSVPVSVKDKDSQG

• Full Name: CD6 antigen
• Calculated Molecular Weight: 72kDa
• GenBank Accession Number: NM_009852.3
• Gene Symbol: Cd6
• Gene ID (NCBI): 12511
• RRID: AB_3748767
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q61003-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.