Anti-Human CD61 / Integrin beta 3 Rabbit Recombinant Antibody

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• Anti-Human CD61 / Integrin beta 3 Rabbit Recombinant Antibody (98864-1-RR)

CD61 / Integrin beta 3 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98864-1-RR

Clone No.252741E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

Integrin beta 3, ITGB3, CD61, GP3A, GPIIIa

• 

  IHC staining of human spleen using 98864-1-RR

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 98864-1-RR (ITB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human platelets using 98864-1-RR

  1x10^6 human platelets were surface stained with 0.25 ug Anti-Human CD61 / Integrin beta 3 Rabbit RecAb (98864-1-RR, Clone: 252741E11) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human platelets using 98864-1-RR

  1x10^6 human platelets were surface stained with 0.25 ug Anti-Human CD61 / Integrin beta 3 Rabbit RecAb (98864-1-RR, Clone: 252741E11) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: human platelets

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98864-1-RR targets CD61 / Integrin beta 3 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10480

  Product name: Recombinant human ITB3 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011435 of

  Sequence: \

• Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Gene Symbol: Integrin beta 3
• Gene ID (NCBI): 3690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.