CoraLite® Plus 647 Anti-Human CD61 / Integrin beta 3 Rabbit Recombinant Antibody

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• CoraLite® Plus 647 Anti-Human CD61 / Integrin beta 3 Rabbit Recombinant Antibody (CL647-98864)

CD61 / Integrin beta 3 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL647-98864

Clone No.252741E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Integrin beta 3, ITGB3, CD61, GP3A, GPIIIa

• 

  FC experiment of human peripheral blood platelets using CL647-98864

  1x10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD61 / Integrin beta 3 Rabbit RecAb (CL647-98864, Clone: 252741E11) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98864 targets CD61 / Integrin beta 3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10480

  Product name: Recombinant human ITB3 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011435 of

  Sequence: \

• Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Gene Symbol: Integrin beta 3
• Gene ID (NCBI): 3690
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.