FcZero-rAb® Biotin Anti-Human P-Selectin/CD62P (AK4) Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood platelets using Biotin-FcA65587

  1x10^6 thrombin-treated human peripheral blood platelets were surface stained with 0.25 ug Biotin Anti-Human CD62P (AK4) Rabbit IgG RecAb (Biotin-FcA65587, Clone: AK4) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9)(blue), and Streptavidin-PE Conjugate (PE-PF00030). 1x10^6 untreated human peripheral blood platelets were surface stained with 0.25 ug Biotin Anti-Human CD62P (AK4) Rabbit IgG RecAb (Biotin-FcA65587, Clone: AK4) and Streptavidin-PE Conjugate (PE-PF00030) (black, dashed). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

P-Selectin/CD62P FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA65587

Clone No.AK4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD62P, P-selectin, AK4, CD62 antigen-like family member P, GMP 140

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Tested Applications

• Positive FC detected in: Thrombin treated human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65587 targets P-Selectin/CD62P in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human platelets

• Full Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Calculated Molecular Weight: 830 aa, 91 kDa
• GenBank Accession Number: BC028067
• Gene Symbol: CD62P
• Gene ID (NCBI): 6403
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16109
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.