PE Anti-Human CD62P (AK4)

CD62P Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

AK4

Cat no : PE-65164

Synonyms

CD62, CD62P, FLJ45155, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, SELP



Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65164 targets CD62P in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen Human platelets
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated molecular weight 830 aa, 91 kDa
GenBank accession numberBC028067
Gene symbol SELP
Gene ID (NCBI) 6403
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496, 565 nm/578 nm
Form Liquid
Purification Method The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
FC protocol for PE CD62P antibody PE-65164Download protocol
Standard Protocols
Click here to view our Standard Protocols