Anti-Human CD63 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood platelets using 98318-1-RR

  1x10^6 Thrombin-treated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD63 Rabbit RecAb (98318-1-RR, Clone: 241964E1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Untreated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD63 Rabbit RecAb (98318-1-RR, Clone: 241964E1) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed.

CD63 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98318-1-RR

Clone No.241964E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63

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Tested Applications

• Positive FC detected in: Thrombin treated human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98318-1-RR targets CD63 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD63 molecule
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC002349
• Gene Symbol: CD63
• Gene ID (NCBI): 967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08962
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that regulate cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein translocated to the plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it is a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.