Anti-Human CD64 (10.1), PBS Only

• 

  FC experiment of human PBMCs using 65253-1-Ig (same clone as 65253-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65253-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65253-1-Ig (same clone as 65253-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65253-1-PBS in a different storage buffer formulation.

CD64 Monoclonal Antibody for FC

Cat No. 65253-1-PBS

Clone No.10.1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, monkey, non-human primates

Applications

FC

Synonyms

CD64A, Fc gamma RI, Fc gamma RIA, FCG1, Fc-gamma RI

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65253-1-Ig

Product Information

65253-1-PBS targets CD64 in FC applications and shows reactivity with human, monkey, non-human primates samples.

• Tested Reactivity: human, monkey, non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

• Full Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Calculated Molecular Weight: 374 aa, 43 kDa
• GenBank Accession Number: BC032634
• Gene Symbol: FCGR1A
• Gene ID (NCBI): 2209
• ENSEMBL Gene ID: ENSG00000150337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P12314
• Storage Buffer: PBS Only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.