Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody

CD64 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

10.1

Cat no : 65572-1-MR

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

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Validation Data Gallery

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  FC experiment of human PBMCs using 65572-1-MR

  1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD64 (10.1) Mouse Recombinant Antibody (65572-1-MR, Clone:10.1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), and 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4). Cells were then stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65572-1-MR targets CD64 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
• Full Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Calculated Molecular Weight: 374 aa, 43 kDa
• GenBank Accession Number: BC032634
• Gene Symbol: FCGR1A
• Gene ID (NCBI): 2209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.