Anti-Human CD64 Mouse Recombinant Antibody

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• Anti-Human CD64 Mouse Recombinant Antibody (98751-1-MR)

CD64 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98751-1-MR

Clone No.253541A2

Host / Isotype

Mouse / IgG

Reactivity

human

Applications

FC

Synonyms

CD64A, Fc gamma RI, Fc gamma RIA, FCG1, Fc-gamma RI

• 

  FC experiment of human PBMCs using 98751-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD64 Mouse RecAb (98751-1-MR, Clone: 253541A2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98751-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD64 Mouse RecAb (98751-1-MR, Clone:253541A2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98751-1-MR targets CD64 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Gene Symbol: FCGR1A
• Gene ID (NCBI): 2209
• ENSEMBL Gene ID: ENSG00000150337
• RRID: AB_3746318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12314
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.