CoraLite® Plus 488-conjugated CD64 Recombinant antibody

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  IF Staining of RAW 264.7 using CL488-84518-2

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CoraLite® Plus 488 CD64 antibody (CL488-84518-2, Clone: 241809D6 ) at dilution of 1:200.

CD64 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84518-2

Clone No.241809D6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Synonyms

241809D6, Fcg1, Fc-gamma RI, Fcgr1, FcRI

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84518-2 targets CD64 in IF/ICC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD64 fusion protein Eg2107
• Full Name: Fc receptor, IgG, high affinity I
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: NM_010186.5
• Gene Symbol: Fcgr1
• Gene ID (NCBI): 14129
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26151
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.