Cardinal Red™ Anti-Human CD64 (10.1)

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  FC experiment of human PBMCs using CR-65253

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD64 (CR-65253, Clone:10.1) (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CR-65253

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD64 (CR-65253, Clone:10.1) or unstained. Cells were not fixed. Monocytes were gated.

CD64 Monoclonal Antibody for FC

Cat No. CR-65253

Clone No.10.1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CR-65253 targets CD64 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

• Full Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Calculated Molecular Weight: 374 aa, 43 kDa
• GenBank Accession Number: BC032634
• Gene Symbol: FCGR1A
• Gene ID (NCBI): 2209
• ENSEMBL Gene ID: ENSG00000150337
• RRID: AB_2935236
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P12314
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.