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APC Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC (Intra)

Conjugate

APC Fluorescent Dye

CloneNo.

Y1/82A

Cat no : APC-65187

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD68 (Y1/82A) (APC-65187)

    FC experiment of human PBMCs using APC-65187

    1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD68 (Y1/82A) (APC-65187)

    FC experiment of human PBMCs using APC-65187

    1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65187 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Lysosomal contents of lung macrophages
Full Name CD68 molecule
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
RRIDAB_2883047
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for APC CD68 antibody APC-65187Download protocol
Standard Protocols
Click here to view our Standard Protocols