FcZero-rAb® CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL488-FcA98029)

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  FC experiment of mouse peritoneal macrophages using CL488-FcA98029

  1x10^6 mouse peritoneal macrophages were intracellularly stained with FcZero-rAb® PE Anti-Mouse CD11b (M1/70, and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD68 Rabbit RecAb (CL488-FcA98029, Clone: 230504G5) or FcZero-rAb® CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9). Cells were incubated with MonoZero™ Human/Mouse 3-in-1 Blocking Reagent (PF00029) prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of mouse peritoneal macrophages using CL488-FcA98029

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD68 Rabbit RecAb (CL488-FcA98029, Clone: 230504G5) (red) or FcZero-rAb® CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9) (blue). Cells were incubated with MonoZero™ Human/Mouse 3-in-1 Blocking Reagent (PF00029) prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD68 FcZero-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-FcA98029

Clone No.230504G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

CD68 antigen, gp110, Scard1

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Tested Applications

• Positive FC (Intra) detected in: mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-FcA98029 targets CD68 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0787

  Product name: Recombinant mouse CD68 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 21-291 aa of NM_001291058.1

  Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001291058.1
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31996-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.