PE Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / Mouse IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

Y1/82A

Cat no : PE-65187

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Validation Data Gallery

• 

  FC experiment of human peripheral blood monocytes using PE-65187

  1X10^6 human peripheral blood monocytes were intracellularly stained with 10.00 ul/test PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) (red) or isotype control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive FC detected in: human peripheral blood monocytes

Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65187 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / Mouse IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lysosomal contents of lung macrophages
• Full Name: CD68 molecule
• Calculated molecular weight: 37 kDa
• GenBank accession number: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.