PE Anti-Human CD68 (Y1/82A)

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  FC experiment of human PBMCs using PE-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CD68 Monoclonal Antibody for FC (Intra)

Cat No. PE-65187

Clone No.Y1/82A

Publications(3)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human and More (1)

Applications

IF, FC (Intra)

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 1 publications below

Product Information

PE-65187 targets CD68 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lysosomal contents of lung macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_2883957
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P34810
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Publications

Species	Application	Title
	FC (Intra)	Cancer Sci; Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment; Authors - Anqi Feng
human	IF	Sci Adv; Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis; Authors - Shuwei Hu
human	FC	Int Immunopharmacol; OTUD4-mediated inhibition of YAP1 signaling pathway in ovarian cancer: Implications for macrophage polarization and recruitment; Authors - Mingyue Li